Phosphatidylethanol (PEth) is a generic term for a large group of phospholipids formed from the cell membrane molecule phosphatidylcholine in the presence of ethanol, making them 100% specific for ethanol.
Due to the relatively slow elimination, PEth´s half-life is about 5 days. PEth is generally accepted as the best biomarkers for ethanol consumption and correlated to alcohol consumption over time. Despite years of practis, no false positives have been reported in humans.
Principles of the Method
Phosphatidylethanol is extracted from whole blood by addition extraction solvent containing an internal standard to the sample. After mixing and centrifugation the supernatant is injected in the LC-MS/MS system, where components are separated on a reversed phase (C8) column using a binary gradient.
redhot diagnostics utilize proprietary technology for the production of PEth Calibrators and a D5-PEth internal standards. Patent EPO 2993434, US 9499572 and 9784701
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